a snapshot assay for the rapid and simple detection of four common hotspot codon mutations in the pik3ca gene快照分析四种常见热点的快速和简单的检测密码子pik3ca基因的突变.pdfVIP
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a snapshot assay for the rapid and simple detection of four common hotspot codon mutations in the pik3ca gene快照分析四种常见热点的快速和简单的检测密码子pik3ca基因的突变
BMC Research Notes BioMed Central
Technical Note Open Access
A SNaPshot assay for the rapid and simple detection of four
common hotspot codon mutations in the PIK3CA gene
1 2 3
Carolyn D Hurst , Tahlita CM Zuiverloon , Christian Hafner ,
2 1
Ellen C Zwarthoff and Margaret A Knowles*
Address: 1Cancer Research UK Clinical Centre, Leeds Institute of Molecular Medicine, St Jamess University Hospital, Leeds, UK, 2Department of
Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, the Netherlands and 3Department of Dermatology, University of Regensburg,
93042 Regensburg, Germany
Email: Carolyn D Hurst - c.hurst@leeds.ac.uk; Tahlita CM Zuiverloon - t.zuiverloon@erasmusmc.nl;
Christian Hafner - christian.hafner@klinik.uni-regensburg.de; Ellen C Zwarthoff - e.zwarthoff@erasmusmc.nl;
Margaret A Knowles* - m.a.knowles@leeds.ac.uk
* Corresponding author
Published: 29 April 2009 Received: 3 October 2008
Accepted: 29 April 2009
BMC Research Notes 2009, 2:66 doi:10.1186/1756-0500-2-66
This article is available from: /1756-0500/2/66
© 2009 Knowles et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Activating mutations in the PIK3CA gen
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