a survey of orphan enzyme activities一个孤儿酶活动的调查.pdfVIP

BMC Bioinformatics BioMed Central Research article Open Access A survey of orphan enzyme activities Yannick Pouliot 1,2 and Peter D Karp*1 Address: 1Bioinformatics Research Group, Artificial Intelligence Center, SRI International, 333 Ravenswood Ave, Menlo Park, California, 94025- 3493, USA and 2Lane Medical Library and Knowledge Management Center, Information Resources and Technology, Stanford University Medical Center, 300 Pasteur Drive. Stanford, CA 94305-5123, USA Email: Yannick Pouliot - ypouliot@; Peter D Karp* - pkarp@ * Corresponding author Published: 10 July 2007 Received: 22 March 2007 Accepted: 10 July 2007 BMC Bioinformatics 2007, 8:244 doi:10.1186/1471-2105-8-244 This article is available from: /1471-2105/8/244 © 2007 Pouliot and Karp; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Using computational database searches, we have demonstrated previously that no gene sequences could be found for at least 36% of enzyme activities that have been assigned an Enzyme Commission number. Here we present a follow-up literature-based survey involving a statistically significant sample of such orphan activities. The survey was intended to determine whether sequences for these enzyme activities are truly unknown, or whether these sequences are