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a useful approach to total analysis of risc-associated rna一个有用的方法来分析risc-associated总rna
BMC Research Notes BioMed Central
Short Report Open Access
A useful approach to total analysis of RISC-associated RNA
†1 †1,2 2
Yukinobu Hayashida* , Takahiro Nishibu , Kunio Inoue and
Tsutomu Kurokawa 1
Address: 1Genome Research Laboratories, Wako Pure Chemical Industries, Ltd. Takada 6-1, Amagasaki, Hyogo 661-0963, Japan and 2Department
of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodaicho, Nadaku, Kobe 657-8501, Japan
Email: Yukinobu Hayashida* - hayashida.yukinobu@wako-chem.co.jp; Takahiro Nishibu - nishibu.takahiro@wako-chem.co.jp;
Kunio Inoue - kunio@kobe-u.ac.jp; Tsutomu Kurokawa - kurokawa.tsutomu@wako-chem.co.jp
* Corresponding author †Equal contributors
Published: 26 August 2009 Received: 14 July 2009
Accepted: 26 August 2009
BMC Research Notes 2009, 2:169 doi:10.1186/1756-0500-2-169
This article is available from: /1756-0500/2/169
© 2009 Hayashida et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Identifying the endogenous RNA induced silencing complex(RISC)-associated RNAs
is essential for understanding the cellular regulatory networks by miRNAs. Recently, isolation of
RISC-associated mRNAs using antibody
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