原创力文档accpbfret an imagej plugin for semi-automatic, fully corrected analysis of acceptor photobleaching fret imagesaccpbfret半自动imagej插件,完全纠正受体分析光漂白烦恼图像.pdfVIP
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accpbfret an imagej plugin for semi-automatic, fully corrected analysis of acceptor photobleaching fret imagesaccpbfret半自动imagej插件,完全纠正受体分析光漂白烦恼图像
BMC Bioinformatics BioMed Central
Software Open Access
AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected
analysis of acceptor photobleaching FRET images
János Roszik, János Szöllősi and György Vereb*
Address: Department of Biophysics and Cell Biology, Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary
Email: János Roszik - janosr@dote.hu; János Szöllősi - szollo@dote.hu; György Vereb* - vereb@dote.hu
* Corresponding author
Published: 19 August 2008 Received: 23 April 2008
Accepted: 19 August 2008
BMC Bioinformatics 2008, 9:346 doi:10.1186/1471-2105-9-346
This article is available from: /1471-2105/9/346
© 2008 Roszik et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: The acceptor photobleaching fluorescence resonance energy transfer (FRET)
method is widely used for monitoring molecular interactions in cells. This method of FRET, while
among those with the simplest mathematics, is robust, self-controlled and independent of
fluorophore amounts and ratios.
Results: AccPbFRET is a user-friendly, efficient ImageJ plugin which allows fully corrected, pixel-
wise calculation and detailed, ROI (region of interest)-based analy
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