a systems biology analysis of long and short-term memories of osmotic stress adaptation in fungi系统生物学分析长期和短期记忆的渗透压力适应的真菌.pdfVIP

You et al. BMC Research Notes 2012, 5:258 /1756-0500/5/258 RESEARCH ARTICLE Open Access A systems biology analysis of long and short-term memories of osmotic stress adaptation in fungi 1,7 2 3 4 5 6 Tao You , Piers Ingram , Mette D Jacobsen , Emily Cook , Andrew McDonagh , Thomas Thorne , 3 1 1 1 6 Megan D Lenardon , Alessandro PS de Moura , M Carmen Romano , Marco Thiel , Michael Stumpf , Neil AR Gow3 4 1 8 3* , Ken Haynes , Celso Grebogi , Jaroslav Stark and Alistair JP Brown Abstract Background: Saccharomyces cerevisiae senses hyperosmotic conditions via the HOG signaling network that activates the stress-activated protein kinase, Hog1, and modulates metabolic fluxes and gene expression to generate appropriate adaptive responses. The integral control mechanism by which Hog1 modulates glycerol production remains uncharacterized. An additional Hog1-independent mechanism retains intracellular glycerol for adaptation. Candida albicans also adapts to hyperosmolarity via a HOG signaling network. However, it remains unknown whether Hog1 exerts integral or proportional control over glycerol production in C. albicans. Results: We combined modeling and experimental approaches to study osmotic stress responses in S. cerevisiae and C. albicans. We propose a simple ordinary differential equation (ODE) model that highlights the integral control that Hog1 exerts over glycerol biosynthesis in these species. If integral control arises from a separation of time scales (i.e. rapid HOG activation of glycerol pr