a simple method to assess the oxidative susceptibility of low density lipoproteins一个简单的方法来评估低密度脂蛋白的氧化易感性.pdfVIP

BMC Clinical Pathology (2001) 1:1 /1472-6890/1/1 BMC Clinical Pathology (2001) 1:1 Methodology article A simple method to assess the oxidative susceptibility of low density lipoproteins Adriana E Scoccia, María Silvina Molinuevo, Antonio Desmond McCarthy and Ana María Cortizo* Address: Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina E-mail: Adriana E Scoccia - ascoccia@.ar; María Silvina Molinuevo - silmol@.ar; Antonio Desmond McCarthy - mccarthy@.ar; Ana María Cortizo* - cortizo@.ar *Corresponding author Published: 20 June 2001 Received: 9 March 2001 Accepted: 20 June 2001 BMC Clinical Pathology 2001, 1:1 This article is available from: /1472-6890/1/1 (c) 2001 Scoccia et al, licensee BioMed Central Ltd. Abstract Background: Oxidative modification of low density lipoproteins (LDL) is recognized as one of the major processes involved in atherogenesis. The in vitro standardized measurement of LDL oxidative susceptibility could thus be of clinical significance. The aim of the present study was to establish a method which would allow the evaluation of oxidative susceptibility of LDL in the general clinical laboratory. Results: LDL was isolated from human plasma by selective precipitation with amphipathic polymers. The ability of LDL to form peroxides was assessed by measuring thiobarbituric acid reactive substances (TBARS) after incubation with Cu2+ and H O . Reaction kinetics showed a 2 2