acute toxicity, brine shrimp cytotoxicity and relaxant activity of fruits of callistemon citrinus curtis急性毒性,盐水虾水果的细胞毒性和放松的活动callistemon citrinus柯蒂斯.pdfVIP

Ali et al. BMC Complementary and Alternative Medicine 2011, 11:99 /1472-6882/11/99 RESEARCH ARTICLE Open Access Acute toxicity, brine shrimp cytotoxicity and relaxant activity of fruits of callistemon citrinus curtis 1,2*† 2 2 2 2 3 Niaz Ali , Ghayour Ahmed , Syed Wadood Ali Shah , Ismail Shah , Mehreen Ghias and Imran Khan Abstract Background: Callistemon citrinus Curtis belongs to family Myrtaceae that has a great medicinal importance. In our previous work, fruits of Callistemon citrinus were reported to have relaxant (antispasmodic) activity. The current work describes the screening of fractions of the crude methanol extract for tracing spasmolytic constituents so that it shall help us for isolation of bioactive compounds. Acute toxicity and brine shrimp cytotoxicity of crude methanol extract are also performed to standardize it. Methods: The crude methanol extract was obtained by maceration with distilled water (500 ml) three times and fractionated successively with n-hexane, chloroform, ethyl acetate and n-butanol (300 ml of each solvent). Phytochemical analysis for crude methanol extract was performed. Acute toxicity studies were performed in mice. Brine shrimp cytotoxicity studies were performed to determine its cytotoxicity and standardize it. In other series of experiments, rabbits’ jejunum preparations were used in screening for possible relaxant activities of various fractions. They were applied in concentrations of 0.01, 0.03, 0.1, 0.3, 1.0, 3.0, 5.0 and 10.0 mg/ml on spontaneous rabbits’ jejunum preparations. In similar fashion, fractions were also tested on KCl (80 mM) -induced contractions. Calcium chloride curves were constructed in K-rich Tyrode’s so