age-dependent alterations of monocyte subsets and monocyte-related chemokine pathways in healthy adults年龄相关性变化的单核细胞和monocyte-related趋化因子通路子集在健康成年人.pdfVIP

Seidler et al. BMC Immunology 2010, 11:30 /1471-2172/11/30 R E S E A R C H A R T I C L E Open Access Research article Age-dependent alterations of monocyte subsets and monocyte-related chemokine pathways in healthy adults †1 †1 2 1 1 Sebastian Seidler , Henning W Zimmermann , Matthias Bartneck , Christian Trautwein and Frank Tacke* Abstract Background: Recent experimental approaches have unraveled essential migratory and functional differences of monocyte subpopulations in mice. In order to possibly translate these findings into human physiology and pathophysiology, human monocyte subsets need to be carefully revisited in health and disease. In analogy to murine studies, we hypothesized that human monocyte subsets dynamically change during ageing, potentially influencing their functionality and contributing to immunosenescence. Results: Circulating monocyte subsets, surface marker and chemokine receptor expression were analyzed in 181 healthy volunteers (median age 42, range 18-88). Unlike the unaffected total leukocyte or total monocyte counts, non- classical CD14+CD16+ monocytes significantly increased with age, but displayed reduced HLA-DR and CX CR1 surface 3 expression in the elderly. Classical CD14++ - CD16 monocyte counts did not vary dependent on age. Serum MCP-1 (CCL2), but not MIP1α (CCL3), MIP1β (CCL4) or fractalkine (CX3CL1) concentrations increased with age. Monocyte- derived macrophages from old or young individuals did not differ with respect to cytokine release in vitro at steady