amiloride derivatives enhance insulin release in pancreatic islets from diabetic mice阿米洛利衍生品提高糖尿病小鼠胰岛细胞的胰岛素释放.pdfVIP

BMC Endocrine Disorders BioMed Central Research article Open Access Amiloride derivatives enhance insulin release in pancreatic islets from diabetic mice Subhadra C Gunawardana, W Steven Head and David W Piston* Address: Department of Molecular Physiology and Biophysics, 702 Light Hall, Vanderbilt University, Nashville, TN 37232, USA Email: Subhadra C Gunawardana - subhadra.gunawardana@; W Steven Head - Steven.Head@; David W Piston* - Dave.Piston@ * Corresponding author Published: 08 December 2005 Received: 30 June 2005 Accepted: 08 December 2005 BMC Endocrine Disorders 2005, 5:9 doi:10.1186/1472-6823-5-9 This article is available from: /1472-6823/5/9 © 2005 Gunawardana et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Amiloride derivatives, commonly used for their diuretic and antihypertensive properties, can also cause a sustained but reversible decrease of intracellular pH (pHi). Using dimethyl amiloride (DMA) on normal rodent pancreatic islets, we previously demonstrated the critical influence of islet pHi on insulin secretion. Nutrient-stimulated insulin secretion (NSIS) requires a specific pHi-range, and is dramatically enhanced by forced intracellular acidification with