an always correlated gene expression landscape for ovine skeletal muscle, lessons learnt from comparison with an “equivalent” bovine landscape一个总是为绵羊的骨骼肌相关基因表达景观,教训比较u201c等效u201d牛景观.pdfVIP

Sun et al. BMC Research Notes 2012, 5:632 /1756-0500/5/632 SHORT REPORT Open Access An Always Correlated gene expression landscape for ovine skeletal muscle, lessons learnt from comparison with an “equivalent” bovine landscape 1,2 2,3 2,3 2 3 3 Wei Sun , Nicholas J Hudson , Antonio Reverter , Ashley J Waardenberg , Ross L Tellam , Tony Vuocolo , Keren Byrne3 and Brian P Dalrymple2,3* Abstract Background: We have recently described a method for the construction of an informative gene expression correlation landscape for a single tissue, longissimus muscle (LM) of cattle, using a small number (less than a hundred) of diverse samples. Does this approach facilitate interspecies comparison of networks? Findings: Using gene expression datasets from LM samples from a single postnatal time point for high and low muscling sheep, and from a developmental time course (prenatal to postnatal) for normal sheep and sheep exhibiting the Callipyge muscling phenotype gene expression correlations were calculated across subsets of the data comparable to the bovine analysis. An “Always Correlated” gene expression landscape was constructed by integrating the correlations from the subsets of data and was compared to the equivalent landscape for bovine LM muscle. Whilst at the high level apparently equivalent modules were identified in the two species, at the detailed level overlap between genes in the equivalent modules was limited and generally not significant. Indeed, only 395 genes and 18 edges were in common between the two landscapes. Conclusions: Since it is unlikely that the equivalent muscles of two closely related species are as different as this analysis suggests, within tissue gene expressi