application of bioinformatics-coupled experimental analysis reveals a new transport-competent nuclear localization signal in the nucleoprotein of influenza a virus strain应用bioinformatics-coupled实验分析揭示了一个新的transport-competent核核蛋白质的定位信号甲型流感病毒株.pdfVIP

BMC Cell Biology BioMed Central Research article Open Access Application of bioinformatics-coupled experimental analysis reveals a new transport-competent nuclear localization signal in the nucleoprotein of Influenza A virus strain Krishna Mohan V Ketha* and Chintamani D Atreya Address: Section of Cell Biology, Laboratory of Cellular Hematology, Division of Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research (F.D.A.) Bethesda, MD 20892, USA Email: Krishna Mohan V Ketha* - krishna.ketha@; Chintamani D Atreya - chintamani.atreya@ * Corresponding author Published: 28 April 2008 Received: 18 December 2007 Accepted: 28 April 2008 BMC Cell Biology 2008, 9:22 doi:10.1186/1471-2121-9-22 This article is available from: /1471-2121/9/22 © 2008 Ketha and Atreya; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Two nuclear localization sequences (NLS) in influenza A virus nucleoprotein (NP) have been demonstrated to be critical for nuclear import of NP and viral ribonucleoprotein complexes. However, a deletion mutant lacking these two signals was still able to localize to the nucleus suggesting the presence of yet another (a third) potential NLS in the NP protein. In order to identify the na