原创力文档assessment of a six gene panel for the molecular detection of circulating tumor cells in the blood of female cancer patients评估六个基因面板的分子检测血液中循环肿瘤细胞的女性癌症患者.pdfVIP
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assessment of a six gene panel for the molecular detection of circulating tumor cells in the blood of female cancer patients评估六个基因面板的分子检测血液中循环肿瘤细胞的女性癌症患者
Obermayr et al. BMC Cancer 2010, 10:666
/1471-2407/10/666
RESEARCH ARTICLE Open Access
Assessment of a six gene panel for the molecular
detection of circulating tumor cells in the blood
of female cancer patients
1,8* 2 1 1 3
Eva Obermayr , Fatima Sanchez-Cabo , Muy-Kheng M Tea , Christian F Singer , Michael Krainer ,
Michael B Fischer4 5 1 6 7 1
, Jalid Sehouli , Alexander Reinthaller , Reinhard Horvat , Georg Heinze , Dan Tong ,
Robert Zeillinger1,8
Abstract
Background: The presence of circulating tumor cells (CTC) in the peripheral blood of cancer patients has been
described for various solid tumors and their clinical relevance has been shown. CTC detection based on the
analysis of epithelial antigens might be hampered by the genetic heterogeneity of the primary tumor and loss of
epithelial antigens. Therefore, we aimed to identify new gene markers for the PCR-based detection of CTC in
female cancer patients.
Methods: Gene expression of 38 cancer cell lines (breast, ovarian, cervical and endometrial) and of 10 peripheral
blood mononuclear cell (PBMC) samples from healthy female donors was measured using microarray technology
(Applied Biosystems). Differentially expressed genes were identified using the maxT test and the 50% one-sided
trimmed maxT-test. Confirmatory RT-qPCR was performed for 380 gene targets using the AB TaqMan® Low Density
Arrays. Then, 93 gene targets were analyzed using the same RT-qPCR platform in tumor tissues of 126 patients with
primary breast, ovarian or endometrial cancer. Finally, blood samples from 26 healthy women and from 125 patients
(primary breast, ov
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