原创力文档assessment of flavonoids contents and in vitro antioxidant activity of launaea procumbens评估内容和类黄酮的体外抗氧化活性的launaea procumbens.pdfVIP
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assessment of flavonoids contents and in vitro antioxidant activity of launaea procumbens评估内容和类黄酮的体外抗氧化活性的launaea procumbens
Khan et al. Chemistry Central Journal 2012, 6:43
/content/6/1/43
RESEARCH ARTICLE Open Access
Assessment of flavonoids contents and in vitro
antioxidant activity of Launaea procumbens
1,2* 2 3 1
Rahmat Ali Khan , Muhammad Rashid Khan , Sumaira Sahreen and Mushtaq Ahmed
Abstract
Background: Launaea procumbens (LP) has been used as a food supplement in Pakistan. In this study methanolic
crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and
chloroform (LPCE) were studied for the determination of total flavonoid and phenolics contents along with
multifaceted in vitro scavenging assays.
Results: Considerable amount of flavonoid and phenolics contents were found in all the fractions. Methanol and
chloroform fraction exhibited efficient scavenging of DPPH•, ABTS•+, •OH, superoxide, lipid peroxide and nitric oxide free
radicals. Significant correlation was found between DPPH•, ABTS•+, superoxide radical, β-carotene bleaching restraint
and phosphomolybdenum assay with total flavonoids and phenolics contents. High performance chromatography
(HPLC) of LPME revealed the presence of vitexin, orientin, rutin, hyperoside, catechin and myricetin.
Conclusion: These results reveal the presence of bioactive compounds in LPME, which might be contributed towards
the various in vitro scavenging.
Keywords: Launaea procumbens, Scavenging of DPPH-free radicals, Superoxide radicals, HPLC, Flavonoids
Background gen atom or an electron, chelating metal catalysts, activat-
Reactive oxygen species (ROS) are generated in the nor- ing antioxidant enzymes and inhibiting ox
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