association of chemerin mrna expression in human epicardial adipose tissue with coronary atherosclerosis协会chemerin mrna表达在人类心外膜脂肪组织与冠状动脉粥样硬化.pdfVIP

Gao et al. Cardiovascular Diabetology 2011, 10:87 CARDIO /content/10/1/87 VASCULAR DIABETOLOGY ORIGINAL INVESTIGATION Open Access Association of chemerin mRNA expression in human epicardial adipose tissue with coronary atherosclerosis 1 2 1 3 4 4 2 Xiuying Gao , Shuhua Mi , Fuzhuang Zhang , Fengying Gong , Yongqiang Lai , Feng Gao , Xiaoxia Zhang , Linjie Wang3 and Hong Tao1* Abstract Background: Growing evidence suggests that epicardial adipose tissue (EAT) may play a key role in the pathogenesis and development of coronary artery disease (CAD) by producing several inflammatory adipokines. Chemerin, a novel adipokine, has been reported to be involved in regulating immune responses and glucolipid metabolism. Given these properties, chemerin may provide an interesting link between obesity, inflammation and atherosclerosis. In this study, we sought to determine the relationship of chemerin expression in EAT and the severity of coronary atherosclerosis in Han Chinese patients. Methods: Serums and adipose tissue biopsies (epicardial and thoracic subcutaneous) were obtained from CAD (n = 37) and NCAD (n = 16) patients undergoing elective cardiac surgery. Gensini score was used to assess the severity of CAD. Serum levels of chemerin, adiponectin and insulin were measured by ELISA. Chemerin protein expression in adipose tissue was detected by immunohistochemistry. The mRNA levels of chemerin, chemR23, adiponectin and TNF-alpha in adipos