b lymphocyte intestinal homing in inflammatory bowel diseaseb淋巴细胞在炎症性肠病肠道归航.pdfVIP

Defendenti et al. BMC Immunology 2011, 12:71 /1471-2172/12/71 RESEARCH ARTICLE Open Access B Lymphocyte intestinal homing in inflammatory bowel disease 1* 2 1 3 3 Caterina Defendenti , Piercarlo Sarzi-Puttini , Silvia Grosso , Annamaria Croce , Olivia Senesi, Simone Saibeni , 4 5 3 2,6 Simona Bollani , Piero Luigi Almasio , Savino Bruno and Fabiola Atzeni Abstract Background: Inflammatory bowel disease (IBD) is thought to be due to an abnormal interaction between the host immune system and commensal microflora. Within the intestinal immune system, B cells produce physiologically natural antibodies but pathologically atypical anti-neutrophil antibodies (xANCAs) are frequently observed in patients with IBD. The objective is to investigate the localisation of immunoglobulin-producing cells (IPCs) in samples of inflamed intestinal tissue taken from patients with IBD, and their possible relationship with clinical features. Methods: The IPCs in small intestinal, colonic and rectal biopsy specimens of patients with IBD were analysed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The B cell phenotype of the IPC-positive samples was assessed using monoclonal antibodies specific for CD79, CD20, CD23, CD21, CD5, l and chains. Statistical correlations were sought between the histological findings and clinical expression. Results: The study involved 96 patients (64 with ulcerative colitis and 32 with Crohn’s disease). Two different patterns of B lymphocyte infiltrates were found in the intestinal tissue: one was characterised by a strong to