contribution of histone sequence preferences to nucleosome organization proposed definitions and methodology贡献的组蛋白序列偏好核小体组织提出的定义和方法.pdfVIP

Kaplan et al. Genome Biology 2010, 11:140 /2010/11/11/140 OPINION Contribution of histone sequence preferences to nucleosome organization: proposed denitions and methodology 1 2,3 4 5 1,6 Noam Kaplan , Timothy R Hughes , Jason D Lieb , Jonathan Widom * and Eran Segal * averaged over a cell population. Another issue is that, Abstract despite intensive research, the terminology and analysis We propose denitions and procedures for comparing methods used in the field vary, leading to ambiguity and nucleosome maps and discuss current agreement confusion. We believe that this has created an incorrect and disagreement on the eect of histone sequence appearance of a major controversy in the field, with preferences on nucleosome organization in vivo. seemingly contradictory paper titles such as ‘A genomic code for nucleosome positioning’ [9], ‘e DNA-encoded nucleosome organization of a eukaryotic genome’ [10], ‘A e organization of nucleosomes in living cells is non- high-resolution, nucleosome position map of C. elegans random and conserved across similar cells [1], and it reveals a lack of universal sequence-dictated positioning’ affects several processes, most notably transcription [11], and ‘Intrinsic histone-DNA interactions are not the [2-5]. It is therefore important to understand what factors major determi