high-performance liquid chromatographic quantification of flavonoids in eriocaulaceae species and their antimicrobial activity高效液相色谱量化谷精草科物种及其抗菌活性的黄酮类化合物.pdfVIP

high-performance liquid chromatographic quantification of flavonoids in eriocaulaceae species and their antimicrobial activity高效液相色谱量化谷精草科物种及其抗菌活性的黄酮类化合物.pdf

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high-performance liquid chromatographic quantification of flavonoids in eriocaulaceae species and their antimicrobial activity高效液相色谱量化谷精草科物种及其抗菌活性的黄酮类化合物

Molecules 2009, 14, 4644-4654; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity 1 2 1 Marcelo Aparecido da Silva , Claudia Andréa Lima Cardoso , Wagner Vilegas and Lourdes Campaner dos Santos 1,* 1 UNESP-Univ Estadual Paulista, Instituto de Química, Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São Paulo, Brazil 2 UEMS, Course of Chemistry, Mato Grosso do Sul State University, CP 351, CEP 79804-970, Dourados, Mato Grosso do Sul, Brazil * Author to whom correspondence should be addressed; E-Mail: loursant@iq.unesp.br; Tel.: +55-16-3301-6657; Fax: +55-16-3301-6692. Received: 9 October 2009; in revised form: 13 November 2009 / Accepted: 16 November 2009 / Published: 16 November 2009 Abstract: Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3%

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