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Pharmaceuticals 2011, 4, 782-793; doi:10.3390/ph4060782 OPEN ACCESS pharmaceuticals ISSN 1424-8247 /journal/pharmaceuticals Article Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells Hiroki Miyashita 1, Hirotada Suzuki 1,2, Akihide Ohkuchi 2 and Yasufumi Sato 1,* 1 Department of Vascular Biology, Institute of Development Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan 2 Department of Obstetrics and Gynecology, Jichi Medical University School of Medicine, Japan * Author to whom correspondence should be addressed; E-Mail: y-sato@idac.tohoku.ac.jp; Tel.: +81-22-717-8528; Fax: +81-22-717-8533. Received: 25 April 2011; in revised form: 23 May 2011/ Accepted: 30 May 2011 / Published: 31 May 2011 Abstract: Vasohibin-1 (VASH1) is a VEGF-inducible gene of endothelial cells (ECs) that acts as a negative feedback regulator of angiogenesis. To further characterize the function of VASH1, we transfected human VASH1 gene into the mouse EC line MS1, established stable VASH1 expressing clones, and determined gene alteration by cDNA microarray analysis. Among the various angiogenesis-related genes, vascular endothelial growth factor type 1 receptor (VEGFR-1) and its alternative spliced form, soluble VEGFR1 (sVEGFR-1), were found to be the most significantly down-regulated genes. Transient overexpression of VASH1 in human umbilical vein endothelial cells confirmed the down-regulation of VEGFR-1 and sVEGFR-1. sVEGFR-1 is a decoy receptor