osmotic response tests on erythrocytes for the detection of double muscle carriers in cattle检测红细胞的渗透反应测试肌肉运营商牛的两倍.pdfVIP

NOTE OSMOTIC RESPONSE TESTS ON ERYTHROCYTES FOR THE DETECTION OF DOUBLE MUSCLE CARRIERS IN CATTLE 1)( W. A. KING, PARVATHI K. BASRUR, R. G. BROWN R T. BERG Dpt. of Biomedical Science, O. V. C., University of Guelph, * Dpt. of Animal and Poultry Science, University of Guelph, Ontario, Canada Dpt.**of Animal science, University of Alberta, Edmonton, Canada SUMMARY Erythrocyte harvested from heparinized whole blood of a group of cattle of various breeds including overtly- double muscled animals and more or less normal carriers of the trait revealed an increased rate of hemolysis on exposure to osmotic shock as compared to normal Charolais cattle. There is also a clearcut distinction between the rate of hemolysis of double muscled animals and normal carriers detected because they have some affinity with the double muscled and have transmitted the trait. They all seem in our samples to be heterozygotes in the generally admitted hypothesis that double muscling is due to a simple mendelian factor. It is hence suggested that the erythrocyte fragility test described in this report may be used for the identification of carriers which are often difficult to distinguish from normal animals on the basis of morphologic criteria. Double muscling syndrome (hereditary muscular hypertrophy) characterized by massive muscles, reduced subcutaneous and intramuscular fat deposits, thin skin and light bones, has been reported to be a