osteoblastogenesis from synovial fluid-derived cells is related to the type and severity of juvenile idiopathic arthritisosteoblastogenesis滑膜fluid-derived细胞与青少年特发性关节炎的类型和严重性.pdfVIP

Lazić et al. Arthritis Research Therapy 2012, 14:R139 /content/14/3/R139 RESEARCH ARTICLE Open Access Osteoblastogenesis from synovial fluid-derived cells is related to the type and severity of juvenile idiopathic arthritis * Elvira Lazić , Marija Jelušić, Danka Grčević, Ana Marušić and Nataša Kovačić Abstract Introduction: Juvenile idiopathic arthritis (JIA) is characterized by synovial inflammation, followed by hyperplastic changes of the synovium, and destruction of articular cartilage along with underlying bone. This hyperplastic process is the result of inflammation-induced activation of NF-B, which may be accompanied by decreased osteogenic differentiation of synovial mesenchymal progenitors and contribute to bone resorption. We aimed to explore osteoblast differentiation of synovial fluid (SF)-derived mesenchymal progenitors and correlate it with intensity of inflammation in patients with JIA. Methods: Peripheral blood from 18 patients with oligoarticular (o)JIA, 22 patients with polyarticular (p)JIA and 18 controls was collected along with SF from 18 patients with oJIA and 9 patients with pJIA. SF-derived cells were cultured to assess osteoblastogenesis, using alkaline phosphatase histochemical staining and colorimetric activity assay. The expression of osteoblast-related genes, Runt-related transcription factor 2 (Runx2), Osteoprotegerin (OPG), Receptor activator of nuclear factor B ligand (RANKL) and arthritis-related cytokine/chemokine genes, Tumor necrosis factor alpha (TNF-a, Fas, Fas ligand (FasL), Interleukin (IL)-1b, IL-4, IL-6, IL-17, IL-18, CC chemokine ligand (CCL)-2, CCL3, CCL4 was evaluated. Osteoblastogenesis was correlated with systemic and local inflammatory indicators. Expression of osteoblast genes was also analyzed in peripheral blood mononuclear cells (PBMC) and