parallel rnai screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology平行rnai屏幕不同细胞系识别通用和细胞肌动蛋白的特定类型的监管机构组织和细胞形态.pdf

Volume 10, Issue 3, Article R26et al.Liu2009 Open Access Research Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology * † * Tao Liu , David Sims and Buzz Baum Addresses: *MRC Laboratory of Molecular Cell Biology, UCL, Gower Street, London WC1E 6BT, UK. †The Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK. Correspondence: Buzz Baum. Email: b.baum@ucl.ac.uk Published: 5 March 2009 Received: 27 November 2008 Revised: 18 February 2009 Genome Biology 2009, 10:R26 (doi:10.1186/gb-2009-10-3-r26) Accepted: 5 March 2009 The electronic version of this article is the complete one and can be found online at /2009/10/3/R26 © 2009 Liu et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. including a neuronal function for the kinase minibrain/Regulation of cell morphologypParallel RNA interference screens and gene expression arrays inDYRK1A in the regulation of protrusion morphology./p six Drosophila cell lines identified regulators of cell morphology, Abstract Background: In recent years RNAi screening has proven a powerful tool for dissecting gene functions in animal cells in culture. However, to date, most RNAi screens have been performed in a single cell line, and