pathogenicity island cag, vaca and is605 genotypes in mexican strains of helicobacter pylori associated with peptic ulcers致病性岛cag,vaca is605基因型在墨西哥株幽门螺杆菌相关的消化性溃疡.pdfVIP

Antonio-Rincón et al. Annals of Clinical Microbiology and Antimicrobials 2011, 10:18 /content/10/1/18 RESEARCH Open Access Pathogenicity island cag, vacA and IS605 genotypes in Mexican strains of Helicobacter pylori associated with peptic ulcers 1 2 2 3 Fernando Antonio-Rincón , Yolanda López-Vidal , Gonzalo Castillo-Rojas , Eduardo C Lazcano-Ponce , Sergio Ponce-de-León5, María L Tabche-Barrera4 and Germán R Aguilar-Gutiérrez1* Abstract Background: Helicobacter pylori is associated with chronic gastritis, peptic ulcers, and gastric cancer. Two major virulence factors of H. pylori have been described: the pathogenicity island cag (cag PAI) and the vacuolating cytotoxin gene (vacA). Virtually all strains have a copy of vacA, but its genotype varies. The cag PAI is a region of 32 genes in which the insertion of IS605 elements in its middle region has been associated with partial or total deletions of it that have generated strains with varying virulence. Accordingly, the aim of this work was to determine the cag PAI integrity, vacA genotype and IS605 status in groups of isolates from Mexican patients with non-peptic ulcers (NPU), non-bleeding peptic ulcers (NBPU), and bleeding peptic ulcers (BPU). Methods: The cag PAI integrity was performed by detection of eleven targeted genes along this locus using dot blot hybridization and PCR assays. The vacA allelic, cag PAI genotype 1 and IS605 status were determined by PCR analysis. Results: Groups of 16-17 isolates (n = 50) from two patients with NPU, NBPU, and BPU, respectively, were studied. 90% (45/50) of the isolates harbored a complete cag PAI. Three BPU isolates lacked the cag PAI, and two of the NBPU had an incomplete cag PAI: t