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phenolic antioxidants isolated from the flowers of osmanthus fragrans酚类抗氧化剂隔离桂花鲜花的.pdf

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phenolic antioxidants isolated from the flowers of osmanthus fragrans酚类抗氧化剂隔离桂花鲜花的

Molecules 2012, 17, 10724-10737; doi:10.3390/molecules170910724 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Phenolic Antioxidants Isolated from the Flowers of Osmanthus fragrans Chien-Ya Hung *, Yu-Cheng Tsai and Kuo-Yu Li Department of Food Nutrition, Chung Hwa University of Medical Technology, No.89, Wenhua 1st St., Rende Dist., Tainan City 71703, Taiwan; E-Mails: renee012783@.tw (Y.-C.T.); lookin.tw (K.-Y.L.) * Author to whom correspondence should be addressed; E-Mail: chienya187@.tw; Tel.: +886-6267-1214 (ext. 705); Fax: +886-6260-5779. Received: 21 July 2012; in revised form: 21 August 2012 / Accepted: 5 September 2012 / Published: 7 September 2012 Abstract: O. fragrans has slightly less antioxidative activity than green tea. Five phenolic compounds, tyrosyl acetate (1), (+)-phillygenin (2), (8E)-ligustroside (3), rutin (4), and verbascoside (5), were isolated from the CHCl sub-extract of O. fragrans. The structures 3 were elucidated by interpreting their spectral data. Evaluation of the antioxidative property of the isolated (+)-phillygenin (2), rutin (4), and verbascoside (5) revealed strong DPPH radical scavenging activity, with IC50 values of 19.1, 10.3, and 6.2 μM, respectively. These isolates also exhibited an H2O2 scavenging ability, with IC50 values of 10.5, 23.4, and 13.4 μM, respectively. Keywords: Osmanthus fragrans; Oleaceae; antioxidative activity;