poly(ethylene glycol)-lipid-conjugated antibodies enhance dendritic cell phagocytosis of apoptotic cancer cells聚(乙二醇)-lipid-conjugated抗体增强树突状细胞吞噬凋亡的肿瘤细胞.pdfVIP

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poly(ethylene glycol)-lipid-conjugated antibodies enhance dendritic cell phagocytosis of apoptotic cancer cells聚(乙二醇)-lipid-conjugated抗体增强树突状细胞吞噬凋亡的肿瘤细胞.pdf

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poly(ethylene glycol)-lipid-conjugated antibodies enhance dendritic cell phagocytosis of apoptotic cancer cells聚(乙二醇)-lipid-conjugated抗体增强树突状细胞吞噬凋亡的肿瘤细胞

Pharmaceuticals 2012, 5, 405-416; doi:10.3390/ph5050405 OPEN ACCESS Pharmaceuticals ISSN 1424-8247 /journal/pharmaceuticals Article Poly(ethylene glycol)-Lipid-Conjugated Antibodies Enhance Dendritic Cell Phagocytosis of Apoptotic Cancer Cells 1 1 1 1 Urara Tomita , Satoshi Yamaguchi , Yoichiro Sugimoto , Satoshi Takamori and Teruyuki Nagamune 1,2,* 1 Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan 2 Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan * Author to whom correspondence should be addressed; E-Mail: nagamune@bio.t.u-tokyo.ac.jp; Tel: +81-3-5841-7356; Fax: +81-3-5841-8657. Received: 8 February 2012; in revised form: 27 March 2012 / Accepted: 17 April 2012 / Published: 26 April 2012 Abstract: A simple method for attaching immunoglobulin G (IgG) on the cell surface was successfully developed for enhancing phagocytosis of apoptotic tumor cells (ATCs) by dendritic cells (DCs) ex vivo. By conjugating with a poly(ethylene glycol) (PEG)-lipid, named the biocompatible anchor for the membrane (BAM), arbitrary IgG could be incorporated into the cell membrane. In particular, when IgG-BAM conjugates were prepared at the optimal molar ratio of IgG to BAM (1 to 20), almost all cells were efficiently