preparation and evaluation of inhalable itraconazole chitosan based polymeric micelles可吸入性伊曲康唑的制备和评价壳聚糖聚合物胶束.pdfVIP

Moazeni et al. DARU Journal of Pharmaceutical Sciences 2012, 20:85 /content/20/1/85 RESEARCH ARTICLE Open Access Preparation and evaluation of inhalable itraconazole chitosan based polymeric micelles 1 1* 1 2 1 Esmaeil Moazeni , Kambiz Gilani , Abdolhossein Rouholamini Najafabadi , Mohamad reza Rouini , Nasir Mohajel , Mohsen Amini3 and Mohammad Ali Barghi4 Abstract Background: This study evaluated the potential of chitosan based polymeric micelles as a nanocarrier system for pulmonary delivery of itraconazole (ITRA). Methods: Hydrophobically modified chitosan were synthesized by conjugation of stearic acid to the hydrophilic depolymerized chitosan. FTIR and 1HNMR were used to prove the chemical structure and physical properties of the depolymerized and the stearic acid grafted chitosan. ITRA was entrapped into the micelles and physicochemical properties of the micelles were investigated. Fluorescence spectroscopy, dynamic laser light scattering and transmission electron microscopy were used to characterize the physicochemical properties of the prepared micelles. The in vitro pulmonary profile of polymeric micelles was studied by an air-jet nebulizer connected to a twin stage impinger. Results: The polymeric micelles prepared in this study could entrap up to 43.2±2.27 μg of ITRA per milliliter. All micelles showed mean diameter between 120–200 nm. The critical micelle concentration of the stearic acid grafted chitosan was found to be 1.58×10-2 mg/ml. The nebulization efficiency was up to 89% and the fine particle fraction (FPF) varied from 38% to 47%. The micelles had enough stability to remain encapsulation of the drug during nebulization process. Conclusions: I