preparative separation of phenolic compounds from halimodendron halodendron by high-speed counter-current chromatography酚类化合物的制备分离从halimodendron halodendron通过高速逆流色谱法.pdf

Molecules 2010, 15, 5998-6007; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Preparative Separation of Phenolic Compounds from Halimodendron halodendron by High-Speed Counter-Current Chromatography Jihua Wang 1, Haifeng Gao 1, Jianglin Zhao 1, Qi Wang 1, Ligang Zhou 1,*, Jianguo Han 2, 2 2 Zhu Yu and Fuyu Yang 1 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China 2 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China * Author to whom correspondence should be addressed; E-Mail: lgzhou@; Tel.: +86 10 Received: 30 July 2010; in revised form: 16 August 2010 / Accepted: 27 August 2010 / Published: 31 August 2010 Abstract: Three phenolic compounds, p -hydroxybenzoic acid (1), isorhamnetin-3-O-β-D- rutinoside (2), and 3,3-di-O-methylquercetin (5), along with a phenolic mixture were successfully separated from the ethyl acetate crude extract of Halimodendron halodendron by high-speed counter-current chromatography (HSCCC) with chloroform-methanol- water-acetic acid (4:3:2:0.05, v/v) as the two-phase solvent system. The phenolic mixture from HSCCC was further separated by preparative HPLC and purified by Sephadex LH-20 to afford quercetin (3) and 3-O-methylquercetin (4). Seven hundred mg of ethyl acetate crude extract was separated by HSCCC to obtain six fractions which were then analyzed by high performance l