production and downstream processing of (1→3)-β-d-glucan from mutant strain of agrobacterium sp. atcc 31750(1→3)的生产和下游加工-β-d-glucan写明atcc 31750。土壤杆菌属sp的突变株.pdf

Kalyanasundaram et al. AMB Express 2012, 2:31 /content/2/1/31 ORIGINAL ARTICLE Open Access Production and downstream processing of (1!3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750 * Gayathiri T Kalyanasundaram, Mukesh Doble and Sathyanarayana N Gummadi Abstract We isolated a mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp. ATCC 31750 by chemical mutagenesis using N-methyl-N-nitro-nitrosoguanidine. The mutant strain produced 66 g/L of curdlan in 120 h with a yield of (0.88) while, the wild strain produced 41 g/L in 120 h with a yield of (0.62) in a stirred bioreactor. The mutant could not produce curdlan when the pH was shifted from 7.0 to 5.5 after nitrogen depletion as followed for wild strain. In contrast, pH optimum for cell growth and curdlan production for mutant was found to be 7.0. We optimized the downstream processing of curdlan by varying different volumes of NaOH and HCl for extraction and precipitation of curdlan. The molecular weight of the purified curdlan from the wild and mutant strain was 6.6 × 105 Da and 5.8 × 105 Da respectively. The monosaccharide analyses confirm that curdlan from both wild and mutant strain contains only glucose units. From the NMR and FTIR data, it has been confirmed that curdlan was exclusively composed of β (1 ! 3)-D-glucan residues. Keywords: Curdlan, Agrobacterium sp. ATCC 31750, Chemical mutagenesis, Two stage culture, Downstream processing, Characterization Introduction the concrete (Lee et al. 1997 Kim et al. 2000). It is also Curdlan is a high molecular weight, water insoluble (alkali used as an immobilization support as it can covalentl