pseudomonas fluorescens alters epithelial permeability and translocates across caco-2tc7 intestinal cells荧光假改变上皮通透性和把caco-2tc7肠道细胞.pdfVIP

Madi et al. Gut Pathogens 2010, 2:16 /content/2/1/16 RESEARCH Open Access Pseudomonas fluorescens alters epithelial permeability and translocates across Caco-2/TC7 intestinal cells 1 2 1 1 1* Amar Madi , Pascal Svinareff , Nicole Orange , Marc GJ Feuilloley , Nathalie Connil Abstract Background: Pseudomonas fluorescens has long been considered as a psychrotrophic microorganism. Recently, we have shown that clinical strains of P. fluorescens (biovar 1) are able to adapt at a growth temperature of 37°C or above and induce a specific inflammatory response. Interestingly, a highly specific antigen of P. fluorescens, I2, is detected in the serum of patients with Crohn’s disease but the possible role of this bacterium in the disease has not yet been explored. In the present study, we examined the ability of a psychrotrophic and a clinical strain of P. fluorescens to modulate the permeability of a Caco-2/TC7 intestinal epithelial model, reorganize the actin cytoskeleton, invade the target cells and translocate across the epithelium. The behaviour of these two strains was compared to that of the well known opportunistic pathogen P. aeruginosa PAO1. Results: Both strains of P. fluorescens were found to decrease the transepithelial resistance (TER) of Caco-2/TC7 differentiated monolayers. This was associated with an increase in paracellular permeability and F-actin microfilaments rearrangements. Moreover, the invasion and translocation tests demonstrated that the two strains used in this study can invade and translocate across the differentiated Caco-2/TC7 cell monolayers. Conclusions: The present work shows for the first time, that P. fluorescens is able to alter the intestinal epithelial barrier function by disorgani