原创力文档quality evaluation of mycelial antrodia camphorata using high-performance liquid chromatography (hplc) coupled with diode array detector and mass spectrometry (dad-ms)质量评价的菌丝体的antrodia camphorata使用高效液相色谱法(hplc)加上二极管阵列检测器和质谱(dad-ms).pdfVIP
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quality evaluation of mycelial antrodia camphorata using high-performance liquid chromatography (hplc) coupled with diode array detector and mass spectrometry (dad-ms)质量评价的菌丝体的antrodia camphorata使用高效液相色谱法(hplc)加上二极管阵列检测器和质谱(dad-ms)
Zhao and Leung Chinese Medicine 2010, 5:4
/content/5/1/4
RESEARCH Open Access
Quality evaluation of mycelial Antrodia
camphorata using high-performance liquid
chromatography (HPLC) coupled with diode array
detector and mass spectrometry (DAD-MS)
*
Sandy Shuo Zhao, Kelvin Sze-Yin Leung
Abstract
Background: Antrodia camphorata (AC) is an important fungus native to Taiwanese forested regions. Scientific
studies have demonstrated that extracts of AC possess a variety of pharmacological functions. This study aims to
identify the full profile fingerprint of nucleosides and nucleobases in mycelial AC and to assess the quality of two
commercial mycelial AC products.
Methods: High-performance liquid chromatography coupled with diode array detector and mass spectrometry
was employed to identify the major components in mycelial AC. The chemical separation was carried out using a
gradient program on a reverse phase Alltima C18 AQ analytical column (250 × 4.6 mm, 5 μm) with the mobile
phase consisting of deionized water and methanol.
Results: Ten nucleosides and nucleobases, two maleimide derivatives, and a sterol were identified as the major
constituents in mycelial AC. These groups of chemical compounds constitute the first chromatographic fingerprint
as an index for quality assessment of this medicinal fungus.
Conclusions: This study provides the first chromatographic fingerprint to assess the quality of mycelial AC.
Background After the success in mass production of AC by artifi-
Antrodia camphorata (M. Zang C.H. Su) Sheng H. cial cultivation, a series of health supplements formu-
Wu, Ryvarden T.T. Chang (Polyporaceae) is a parasi- lated from AC has been lau
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