real-time pcr machine system modeling and a systematic approach for the robust design of a real-time pcr-on-a-chip system实时pcr机器系统建模和系统方法的稳健设计实时pcr-on-a-chip系统.pdfVIP

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real-time pcr machine system modeling and a systematic approach for the robust design of a real-time pcr-on-a-chip system实时pcr机器系统建模和系统方法的稳健设计实时pcr-on-a-chip系统.pdf

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real-time pcr machine system modeling and a systematic approach for the robust design of a real-time pcr-on-a-chip system实时pcr机器系统建模和系统方法的稳健设计实时pcr-on-a-chip系统

Sensors 2010, 10, 697-718; doi:10.3390/s100100697 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System Da-Sheng Lee Department of Energy and Refrigerating Air Conditioning Engineering, National Taipei University of Technology, Taipei, Taiwan; E-Mail: f11167@.tw; Tel.: +886-2-2771-2171 ext 3510; Fax: +886-2-2731-4919 Received: 7 December 2009; in revised form: 8 January 2010 / Accepted: 12 January 2010 / Published: 19 January 2010 Abstract: Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar re