pyrosequencing of 16s rrna gene amplicons to study the microbiota in the gastrointestinal tract of carp (cyprinus carpio l.)焦磷酸测序16 s rrna基因的扩增子研究胃肠道微生物群的鲤鱼(鲤属carpio l .).pdfVIP

van Kessel et al. AMB Express 2011, 1:41 /content/1/1/41 ORIGINAL Open Access Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp (Cyprinus carpio L.) 1,2 3,4 5 5 5 2 Maartje AHJ van Kessel , Bas E Dutilh , Kornelia Neveling , Michael P Kwint , Joris A Veltman , Gert Flik , Mike SM Jetten1*, Peter HM Klaren2 and Huub JM Op den Camp1 Abstract The microbes in the gastrointestinal (GI) tract are of high importance for the health of the host. In this study, Roche 454 pyrosequencing was applied to a pooled set of different 16S rRNA gene amplicons obtained from GI content of common carp (Cyprinus carpio) to make an inventory of the diversity of the microbiota in the GI tract. Compared to other studies, our culture-independent investigation reveals an impressive diversity of the microbial flora of the carp GI tract. The major group of obtained sequences belonged to the phylum Fusobacteria. Bacteroidetes, Planctomycetes and Gammaproteobacteria were other well represented groups of micro-organisms. Verrucomicrobiae, Clostridia and Bacilli (the latter two belonging to the phylum Firmicutes) had fewer representatives among the analyzed sequences. Many of these bacteria might be of high physiological relevance for carp as these groups have been implicated in vitamin production, nitrogen cycling and (cellulose) fermentation. Keywords: intestinal tract, biodiversity, carp, aquaculture, pyrosequencing, 16S rRNA Introduction relevant for aquaculture practice. We investigated the The intestine is a multifunctional organ system involved diversity of the microbiota in