putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones假定的分子机制精子染色质重塑是由生殖激素.pdfVIP

Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones Gill-Sharma et al. Gill-Sharma et al. Clinical Epigenetics 2012, 4:23 /content/4/1/23 Gill-Sharma et al. Clinical Epigenetics 2012, 4:23 /content/4/1/23 RESEARCH Open Access Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones 1* 2 3 4 Manjeet Kaur Gill-Sharma , Jyoti Choudhuri , Mukhtar Aleem Ansari and Serena D’Souza Abstract Background: The putative regulatory role of the male reproductive hormones in the molecular mechanism underlying chromatin condensation remains poorly understood. In the past decade, we developed two adult male rat models wherein functional deficits of testosterone or FSH, produced after treatments with 20 mg/Kg/d of cyproterone acetate (CPA) per os, for a period of 15 days or 3 mg/Kg/d of fluphenazine decanoate (FD) subcutaneously, for a period of 60 days, respectively, affected the rate of sperm chromatin decondensation in vitro. These rat models have been used in the current study in order to delineate the putative roles of testosterone and FSH in the molecular mechanism underlying remodelling of sperm chromatin. Results: We report that deficits of both testosterone and FSH affected the turnover of polyubiquitylated histones and led to their accumulation in the testis. Functional deficits of testosterone reduced expression of MIWI, the 5-methyl cap binding RNA-binding protein (PIWIlike murine homologue of the Drosophila protein PIWI/P-element induced wimpy testis) contain