rna aptamer evolution two decades of selection核糖核酸适体进化二十年的选择.pdfVIP

Int. J. Mol. Sci. 2011, 12, 9155-9171; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Review RNA Aptamer Evolution: Two Decades of SELEction Guillermo Aquino-Jarquin 1 and Julia D. Toscano-Garibay 2,* 1 Unit of Research on Oncological Disease, Children’s Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico; E-Mail: guillaqui@.mx 2 Regenerative Medicine Laboratory. Research Direction, Mexico’s Juarez Hospital, Mexico City 07760, Mexico * Author to whom correspondence should be addressed; E-Mail: julia.toscano@salud.gob.mx; Tel.: +52-55ext.7476. Received: 24 October 2011; in revised form: 24 November 2011 / Accepted: 28 November 2011 / Published: 8 December 2011 Abstract: Aptamers are small non-coding RNAs capable of recognizing, with high specificity and affinity, a wide variety of molecules in a manner that resembles antibodies. This class of nucleic acids is the resulting product of applying a well-established screening method known as SELEX. First developed in 1990, the SELEX process has become a powerful tool to select structured oligonucleotides for the recognition of targets, starting with small molecules, going through protein complexes until whole cells. SELEX has also evolved along with new technologies positioning itself as an alternative in the design of a new class of therapeutic agents