rna-seq based transcriptional map of bovine respiratory disease pathogen “histophilus somni 2336”牛呼吸道疾病病原体rna-seq基础转录地图u201dhistophilus somni 2336u201d.pdfVIP

RNA-Seq Based Transcriptional Map of Bovine Respiratory Disease Pathogen ‘‘Histophilus somni 2336’’ 1,2,3 1,2 4 2 5 Ranjit Kumar , Mark L. Lawrence , James Watt , Amanda M. Cooksey , Shane C. Burgess , Bindu Nanduri1,2* 1 College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, United States of America, 2 Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, Mississippi, United States of America, 3 Center for Clinical and Translational Science, University of Alabama at Birmingham, Birmingham, Alabama, United States of America, 4 Eagle Applied Sciences LLC, San Antonio, Texas, United States of America, 5 College of Agriculture and Life Sciences, The University of Arizona, Tucson, Arizona, United States of America Abstract Genome structural annotation, i.e., identification and demarcation of the boundaries for all the functional elements in a genome (e.g., genes, non-coding RNAs, proteins and regulatory elements), is a prerequisite for systems level analysis. Current genome annotation programs do not identify all of the functional elements of the genome, especially small non-coding RNAs (sRNAs). Whole genome transcriptome analysis is a complementary method to identify ‘‘novel’’ genes, small RNAs, regulatory regions, and operon structures, thus improving the structural annotation in bacteria. In particular, the identification of non-coding RNAs has revealed their widespread occurrence and functional importance in gene regulation, stress and virulence. However, very little is known about non-coding transcripts in Histophilus somni, one of the causative agents of Bovine Respiratory Disease (BRD) as well as bovine infertility, aborti