role of micrornas in controlling gene expression in different segments of the human epididymis小分子核糖核酸的作用在控制人类附睾的基因表达在不同的领域.pdfVIP

Role of MicroRNAs in Controlling Gene Expression in Different Segments of the Human Epididymis ´ ´ 1 ´ 2 ´ 3 4 ´ ´ 1 Clemence Belleannee *, Ezequiel Calvo , Veronique Thimon , Daniel G. Cyr , Christine Legare , Louis Garneau1, Robert Sullivan1,2* ´ ´ ´ ´ ´ ´ ´ 1 Centre de Recherche du CHUQ and Departement d’Obstetrique-Gynecologie, Faculte de Medecine, Universite Laval, Quebec, Canada, 2 Laboratory of Endocrinology ´ ´ ´ ´ and Genomics, CHUL Research Center and Department of Molecular Medicine, Universite Laval, Quebec, Canada, 3 Departement de Biologie, Universite de la Martinique, ´ ´ ´ Martinique, France, 4 INRS-Institut Armand-Frappier, Universite du Quebec, Laval, Quebec, Canada Abstract Background: The molecular mechanisms implicated in regionalized gene expression in the human epididymis have not yet been fully elucidated. Interestingly, more than 200 microRNAs (miRNAs) have been identified in the human epididymis and could be involved in the regulation of mRNA stability and post-transcriptional expression in this organ. Methods: Using a miRNA microarray approach, we investigated the correlation between miRNA signatures and gene expression profiles found in three distinct regions (caput, corpus and cauda) of human epididymides from 3 donors. In silico prediction of transcript miRNA targets was performed using TargetScan and Miranda software’s. FHCE1 immortalized epididymal cell lines were cotransf