基于snp位点对长爪沙鼠apoe基因遗传多态性-中国试验动物学报.doc

研究报告 长爪沙鼠ApoE基因SNPs的遗传多态性 刘月环，王志远1，杜江涛1，余 强1，余陈欢1，吴旧生2，应华忠1 (1.浙江省医学科学院，杭州，310013；2.浙江大学动物科学学院，杭州，310029) 【】 评价ApoE基因在Z:ZCLA长爪沙鼠single nucleotide polymorphism）位点在普通级和清洁级两个封闭群共444只动物中进行了ApoE等位基因的基因频率，基因型频率，杂合度、多态信息量等参数进行了检测与计算。结果　检测结果表明97、781和1774三个SNP位点平均等位基因为2个，遗传方式基本符合孟德尔定律；期望杂合度分别是0.063、0.501、0.499，全群平均为0.354；PIC分别是0.061、0375、0.374，全群平均0.270。　结论　ApoE基因频率和基因型分布可能与长爪沙鼠的长期封闭和选种方式造成一定程度的遗传漂变相关。 【】【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.004.000 Evaluation of genetic polymorphisms of ApoE gene in Mongolian gerbils based on the single nucleotide polymorphisms LIU Yue-huan1, WANG Zhi-yuan1, DU Jiang-tao1, YU Qiang1, YU Chen-huan1, WU Jiu-sheng2, YING Hua-zhong1 Zhejiang Academy of Medical Sciences, Hangzhou 310013, Zhejiang , China;2. College of Animal science, Zhejiang University, Hangzhou 310058） 【Abstract】Objective To evaluate the genetic diversity of ApoE gene in Z: ZCLA gerbil closed. Methods PCR-SSCP technique was employed to calculate the gene frequency, genotype frequency, heterozygosity, polymorphism information content of three SNPs of ApoE gene, which were reported in our previous work, in a total of 444 ordinary and clean Mongolian gerbils. Results The average allele of 3 SNP site 97, 981 and 1774 was 2. The inheritance pattern was in accordance with Mendels law. Expected heterozygosities were 0.063, 0.501, and 0.499, and the average expected heterozygosity of whole group was 0.354. PIC were 0.061, 0375, and 0.374, with an average PIC of 0.270. Conclusions The ApoE gene frequency and genotype distribution in Mongolian gerbils may be to a certain degree associated with the genetic drift caused by long-term closed and selected breeding. 【Key words】Mongolian gerbil; Hyperlipidemia; apolipoprotein E (ApoE) gene; Single nucleotide polymorphisms (SNPs) 高脂血症及脂肪肝是较常见的疾病，主要是由脂代谢紊乱引起，是导致心脑血管疾病重要的危险因子之一[1]。随着人们生活水平的提高及西方饮食文化的渗入，目前该病的发病率在我国发达地区有时高达50%[2-3]，因此研究该病的发生机制，寻找高效的防治靶点，筛选药物，培育和建立具