sperm morphology in estonian and tori breed stallions精子形态在爱沙尼亚和tori品种种马.pdfVIP

Acta vet. scand. 2004, 45, 11-18. Sperm morphology in Estonian and Tori Breed Stallions 1,3 2 1 3 By A. Kavak , N. Lundeheim , M. Aidnik , S.Einarsson 1Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Estonian Agricultural University, Tartu, Estonia, 2Department of Animal Breeding and Genetics, Faculty of Agriculture, Landscape Planning and Horticulture, and 3Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish Uni- versity of Agricultural Sciences, CRU, Uppsala, Sweden. Kavak A, Lundeheim N, Aidnik M, Einarsson S: Sperm morphology in Estonian and Tori Breed stallions. Acta vet. scand. 2004, 45, 11-18. – The standard procedure for assessing the breeding potential of a stallion includes the parameter total number of spermatozoa classified as morphologically normal. This study investigated sperm mor- phology of fresh semen in randomly chosen Estonian (E, n=8) and Tori (T, n=7) breed stallions with proven fertility. Two ejaculates were examined from each stallion. An aliquot from each ejaculate was fixed in 1 mL formol-saline immediately after collec- tion and examined with phase-contrast microscope at a magnification 1000x for all types of morphological abnormalities. Furthermore smears were prepared and stained according to Williams (carbolfuchsin-eosin) for a more detailed examination of the sperm heads with light microscope at a magnification 1000x. Analysis of variance was applied to the data, and results are presented as LSmeans (±SE). One T stallion that had a disturbance in the spermatogenesis and one 22-year-old E stallion were not included in the analyses. The T stallions had