stable gene transfer of ccr5 and cxcr4 sirnas by sleeping beauty transposon system to confer hiv-1 resistance稳定的ccr5基因转移和趋化因子受体cxcr4 sirnas睡美人转座子系统具有抗hiv - 1.pdfVIP

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stable gene transfer of ccr5 and cxcr4 sirnas by sleeping beauty transposon system to confer hiv-1 resistance稳定的ccr5基因转移和趋化因子受体cxcr4 sirnas睡美人转座子系统具有抗hiv - 1.pdf

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stable gene transfer of ccr5 and cxcr4 sirnas by sleeping beauty transposon system to confer hiv-1 resistance稳定的ccr5基因转移和趋化因子受体cxcr4 sirnas睡美人转座子系统具有抗hiv - 1

AIDS Research and Therapy BioMed Central Research Open Access Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance Mayur Tamhane and Ramesh Akkina* Address: Dept. Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, 80523, USA Email: Mayur Tamhane - mayur@; Ramesh Akkina* - akkina@ * Corresponding author Published: 30 July 2008 Received: 25 March 2008 Accepted: 30 July 2008 AIDS Research and Therapy 2008, 5:16 doi:10.1186/1742-6405-5-16 This article is available from: /content/5/1/16 © 2008 Tamhane and Akkina; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Thus far gene therapy strategies for HIV/AIDS have used either conventional retroviral vectors or lentiviral vectors for gene transfer. Although highly efficient, their use poses a certain degree of risk in terms of viral mediated oncogenesis. Sleeping Beauty (SB) transposon system offers a non-viral method of gene transfer to avoid this possible risk. With respect to conferring HIV resistance, stable knock down of HIV-1 coreceptors CCR5 and CXCR4 by the use of lentiviral vector delivered siRNAs has proved to be a promising strategy to protect cells from