synaptic maturation at cortical projections to the lateral amygdala in a mouse model of rett syndrome在皮质突触成熟预测外侧杏仁核在此病的小鼠模型.pdfVIP

Synaptic Maturation at Cortical Projections to the Lateral Amygdala in a Mouse Model of Rett Syndrome ´ ´ 1¤ 1,2,3 1 2 2 Frederic Gambino , Malik Khelfaoui , Bernard Poulain , Thierry Bienvenu , Jamel Chelly , Yann Humeau1,3* ´ ´ ´ 1 Institut des Neurosciences Cellulaires et Integratives, CNRS UPR 3212 et Universite de Strasbourg, Strasbourg, France, 2 Institut Cochin, INSERM Unite 567, CNRS UMR ´ 8104, Paris V, CHU Cochin, Paris, France, 3 Physiologie cellulaire de la synapse, CNRS UMR 5091 et Universite de Bordeaux2, Bordeaux, France Abstract Rett syndrome (RTT) is a neuro-developmental disorder caused by loss of function of Mecp2 - methyl-CpG-binding protein 2 - an epigenetic factor controlling DNA transcription. In mice, removal of Mecp2 in the forebrain recapitulates most of behavioral deficits found in global Mecp2 deficient mice, including amygdala-related hyper-anxiety and lack of social interaction, pointing a role of Mecp2 in emotional learning. Yet very little is known about the establishment and maintenance of synaptic function in the adult amygdala and the role of Mecp2 in these processes. Here, we performed a longitudinal examination of synaptic properties at excitatory projections to principal cells of the lateral nucleus of the amygdala (LA) in Mecp2 mutant mice and their wild-type littermates. We first show that during animal life, Cortico-LA projections switch fro