the agaricus bisporus cox1 gene the longest mitochondrial gene and the largest reservoir of mitochondrial group i introns双孢菇cox1基因最长的线粒体基因和最大的水库线粒体组我内含子.pdfVIP

The Agaricus bisporus cox1 Gene: The Longest Mitochondrial Gene and the Largest Reservoir of Mitochondrial Group I Introns ´ 1 2,3 3 1 1 ´ Cyril Ferandon , Serge Moukha , Philippe Callac , Jean-Pierre Benedetto , Michel Castroviejo , Gerard Barroso1* ´ ` 1 UMR 5234 CNRS (Centre National de la Recherche Scientifique) – Universite Victor Segalen Bordeaux 2, Bordeaux, France, 2 Laboratoire de Toxicologie et Hygiene ´ ´ Appliquee, UFR des Sciences Pharmaceutiques, Universite Victor Segalen Bordeaux 2, Bordeaux, France, 3 INRA (Institut National de la Recherche Agronomique) UR 1264 ´ ´ Mycologie et Securite des Aliments, Villenave d’Ornon, France Abstract In eukaryotes, introns are located in nuclear and organelle genes from several kingdoms. Large introns (up to 5 kbp) are frequent in mitochondrial genomes of plant and fungi but scarce in Metazoa, even if these organisms are grouped with fungi among the Opisthokonts. Mitochondrial introns are classified in two groups (I and II) according to their RNA secondary structure involved in the intron self-splicing mechanism. Most of these mitochondrial group I introns carry a ‘‘Homing Endonuclease Gene’’ (heg) encoding a DNA endonuclease acting in transfer and site-specific integration (‘‘homing’’) and allowing intron spreading and gain after lateral transfer even between species from different kingdoms. Opposed to this gain mechanism, is another which implies that introns, which would have been abundant in the ancestral g