the bzip transcription factor moap1 mediates the oxidative stress response and is critical for pathogenicity of the rice blast fungus magnaporthe oryzaebzip转录因子moap1介导的氧化应激反应和至关重要的稻瘟病菌致病性magnaporthe oryzae.pdfVIP

The bZIP Transcription Factor MoAP1 Mediates the Oxidative Stress Response and Is Critical for Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae 1 1 1 1 1 1 1 1 Min Guo , Yue Chen , Yan Du , Yanhan Dong , Wang Guo , Su Zhai , Haifeng Zhang , Suomeng Dong , 1 1 2 1 Zhengguang Zhang *, Yuanchao Wang , Ping Wang , Xiaobo Zheng 1 Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing, China, 2 Department of Pediatrics and the Research Institute for Children, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America Abstract Saccharomyces cerevisiae Yap1 protein is an AP1-like transcription factor involved in the regulation of the oxidative stress response. An ortholog of Yap1, MoAP1, was recently identified from the rice blast fungus Magnaporthe oryzae genome. We found that MoAP1 is highly expressed in conidia and during invasive hyphal growth. The Moap1 mutant was sensitive to H O , similar to S. cerevisiae yap1 mutants, and MoAP1 complemented Yap1 function in resistance to H O , albeit partially. 2 2 2 2 The Moap1 mutant also exhibited various defects in aerial hyphal growth, mycelial branching, conidia formation, the production of extracellular peroxidases and laccases, and melanin pigmentation. Consequently, the Moap1 mutant was unable to infect the host plant. The MoAP1-eGFP fusion protein