the cyclase-associated protein cap1 is important for proper regulation of infection-related morphogenesis in magnaporthe oryzaecyclase-associated蛋白cap1 magnaporthe感染相关性形态发生的适当监管最重要的是oryzae.pdfVIP

The Cyclase-Associated Protein Cap1 Is Important for Proper Regulation of Infection-Related Morphogenesis in Magnaporthe oryzae 1 1 1,2 3 1,2 Xiaoying Zhou , Haifeng Zhang , Guotian Li , Brian Shaw , Jin-Rong Xu * 1 Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana, United States of America, 2 Purdue-NWAFU Joint Research Center, College of Plant Protection, Northwest AF University, Yangling, Shanxi, China, 3 Department of Plant Pathology and Microbiology, Texas AM University, College Station, Texas, United States of America Abstract Surface recognition and penetration are critical steps in the infection cycle of many plant pathogenic fungi. In Magnaporthe oryzae, cAMP signaling is involved in surface recognition and pathogenesis. Deletion of the MAC1 adenylate cyclase gene affected appressorium formation and plant infection. In this study, we used the affinity purification approach to identify proteins that are associated with Mac1 in vivo. One of the Mac1-interacting proteins is the adenylate cyclase-associated protein named Cap1. CAP genes are well-conserved in phytopathogenic fungi but none of them have been functionally characterized. Deletion of CAP1 blocked the effects of a dominant RAS2 allele and resulted in defects in invasive growth and a reduced intracellular cAMP level. The Dcap1 mutant was defective in germ tube growth, appressorium formation, and formation of typical blast lesions. Cap1-GFP had an actin-like localization pattern, localizing to the apical regions in vegetative hyphae, at the periphery of developing appressoria, and in circular structures at the base of mature appressoria. Interestingly, Cap1, similar to LifeAct, did not localize to the a