the bacterial fimbrial tip acts as a mechanical force sensor细菌fimbrial小费作为机械力传感器.pdfVIP

The Bacterial Fimbrial Tip Acts as a Mechanical Force Sensor 1. 2. 2 3 1 Pavel Aprikian , Gianluca Interlandi , Brian A. Kidd , Isolde Le Trong , Veronika Tchesnokova , Olga 2 2 4 3 2 Yakovenko , Matt J. Whitfield , Esther Bullitt , Ronald E. Stenkamp , Wendy E. Thomas *, Evgeni V. Sokurenko1* 1 Department of Microbiology, University of Washington, Seattle, Washington, United States of America, 2 Department of Bioengineering, University of Washington, Seattle, Washington, United States of America, 3 Departments of Biological Structure and Biochemistry, University of Washington, Seattle, Washington, United States of America, 4 Department of Physiology Biophysics, Boston University School of Medicine, Boston, Massachusetts, United States of America Abstract There is increasing evidence that the catch bond mechanism, where binding becomes stronger under tensile force, is a common property among non-covalent interactions between biological molecules that are exposed to mechanical force in vivo. Here, by using the multi-protein tip complex of the mannose-binding type 1 fimbriae of Escherichia coli, we show how the entire quaternary structure of the adhesive organella is adapted to facilitate binding under mechanically dynamic conditions induced by flow. The fimbrial tip mediates shear-dependent adhesion of bacteria to uroepithelial cells and demonstrates force-enhanced interaction with mannose in single molecule force spectroscopy experiments. The mannose- binding, lec