the discovery of putative urine markers for the specific detection of prostate tumor by integrative mining of public genomic profiles假定的尿液标志物的发现的具体检测前列腺肿瘤的综合矿业公共基因组资料.pdfVIP

The Discovery of Putative Urine Markers for the Specific Detection of Prostate Tumor by Integrative Mining of Public Genomic Profiles 1,3. 2. 1,3 2 1 Min Chen , Kai Wang , Liang Zhang , Cheng Li *, Yongliang Yang * 1 Center for Molecular Medicine, School of Life Science and Biotechnology, Dalian University of Technology, Dalian, People’s Republic of China, 2 Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Harvard School of Public Health, Boston, Massachusetts, United States of America, 3 School of Software, Dalian University of Technology, Dalian, People’s Republic of China Abstract Urine has emerged as an attractive biofluid for the noninvasive detection of prostate cancer (PCa). There is a strong imperative to discover candidate urinary markers for the clinical diagnosis and prognosis of PCa. The rising flood of various omics profiles presents immense opportunities for the identification of prospective biomarkers. Here we present a simple and efficient strategy to derive candidate urine markers for prostate tumor by mining cancer genomic profiles from public databases. Prostate, bladder and kidney are three major tissues from which cellular matters could be released into urine. To identify urinary markers specific for PCa, upregulated entities that might be shed in exosomes of bladder cancer and kidney cancer are first excluded. Through the ontology-based filtering and further assessment, a reduced list of 19 entities encoding urinary proteins was derived as putative PCa markers. Among them, we have found 10 entities closely associated with the process of tumor cell growth and development by pathway enrichment analysis. Further, using the 10 entities as seeds,