the eef1γ subunit contacts rna polymerase ii and binds vimentin promoter regioneef1γ亚基联系人rna聚合酶ii和结合波形蛋白启动子区域.pdfVIP

The eEF1c Subunit Contacts RNA Polymerase II and Binds Vimentin Promoter Region 1 1 1,2 1 3 Nicoletta Corbi , Enrico Maria Batassa , Cinzia Pisani , Annalisa Onori , Maria Grazia Di Certo , 3 4 3 1 Georgios Strimpakos , Maurizio Fanciulli , Elisabetta Mattei , Claudio Passananti * 1 Istituto di Biologia e Patologia Molecolari CNR, c/o Regina Elena Cancer Institute, Rome, Italy, 2 Department of Experimental Medicine, University of L’Aquila, L’Aquila, Italy, 3 Istituto di Neurobiologia e Medicina Molecolare, Consiglio Nazionale delle Ricerche (CNR), IRCCS Fondazione S. Lucia, Rome, Italy, 4 Department of Therapeutic Programs Development, Regina Elena Cancer Institute, Rome, Italy Abstract Here, we show that the eukaryotic translation elongation factor 1 gamma (eEF1c) physically interacts with the RNA polymerase II (pol II) core subunit 3 (RPB3), both in isolation and in the context of the holo-enzyme. Importantly, eEF1c has been recently shown to bind Vimentin mRNA. By chromatin immunoprecipitation experiments, we demonstrate, for the first time, that eEF1c is also physically present on the genomic locus corresponding to the promoter region of human Vimentin gene. The eEF1c depletion causes the Vimentin protein to be incorrectly compartmentalised and to severely compromise cellular shape and mitochondria localisation. We demonstrate that eEF1c partially colocalises with the mitochondrial marker Tom20 and that eEF1c depletion increases mitochondrial superoxide generation as well as the total levels of carbonylated proteins. Finally, we hypothesise that eEF1c, in addition to its role i