the effect of sericin from various extraction methods on cell viability and collagen production丝胶的影响从不同提取方法对细胞生存能力和胶原蛋白的生产.pdfVIP

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the effect of sericin from various extraction methods on cell viability and collagen production丝胶的影响从不同提取方法对细胞生存能力和胶原蛋白的生产.pdf

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the effect of sericin from various extraction methods on cell viability and collagen production丝胶的影响从不同提取方法对细胞生存能力和胶原蛋白的生产

Int. J. Mol. Sci. 2010, 11, 2200-2211; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production Pornanong Aramwit 1,*, Sorada Kanokpanont 2, Titpawan Nakpheng 3 and Teerapol Srichana 3 1 Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 2 Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand; E-Mail: sorada.k@chula.ac.th 3 Department of Pharmaceutical Technology and Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand; E-Mails: titpawan@ (T.N.); teerapol.s@psu.ac.th (T.S.) * Author to whom correspondence should be addressed; E-Mail: aramwit@; Tel.: +66-089-921-7255; Fax: +66-02-218-8403. Received: 16 April 2010; in revised form: 12 May 2010 / Accepted: 13 May 2010 / Published: 20 May 2010 Abstract: Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content. The MTT assay indicated th