the extracytoplasmic stress factor, σe, is required to maintain cell envelope integrity in escherichia coliσe extracytoplasmic压力因素,需要保持在大肠杆菌细胞被膜的完整性.pdfVIP

E The Extracytoplasmic Stress Factor, s , Is Required to Maintain Cell Envelope Integrity in Escherichia coli Jennifer D. Hayden, Sarah E. Ades* Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America Abstract Extracytoplasmic function or ECF sigma factors are the most abundant class of alternative sigma factors in bacteria. Members of the rpoE subclass of ECF sigma factors are implicated in sensing stress in the cell envelope of Gram-negative bacteria and are required for virulence in many pathogens. The best-studied member of this family is rpoE from Escherichia coli, encoding the sE protein. sE has been well studied for its role in combating extracytoplasmic stress, and the members of its regulon have been largely defined. sE is required for viability of E. coli, yet none of the studies to date explain why sE is essential in seemingly unstressed cells. In this work we investigate the essential role of sE in E. coli by analyzing the phenotypes associated with loss of sE E activity and isolating suppressors that allow cells to live in the absence of s . We demonstrate that when sE is inhibited, cell envelope stress increases and envelope integrity is lost. Many cells lyse and some develop blebs containing cytoplasmic material along their sides. To better understand the connection between transcription by sE E and cell envelo