the gammaherpesvirus m2 protein manipulates the fynvav pathway through a multidocking mechanism of assemblygammaherpesvirus m2蛋白操纵fynvav通路通过multidocking组装机制.pdfVIP

The Gammaherpesvirus m2 Protein Manipulates the Fyn/ Vav Pathway through a Multidocking Mechanism of Assembly 1,2. 1,2. 1,2 ´ 1,2 1,2 Marta Pires de Miranda , Marta Alenquer , Sofia Marques , Lenia Rodrigues , Filipa Lopes , ´ 3*, J. Pedro Simas1,2* Xose R. Bustelo ˆ 1 Instituto de Microbiologia e Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal, 2 Instituto Gulbenkian de Ciencia, Oeiras, ´ ´ ´ ´ ´ Portugal, 3 Centro de Investigacion del Cancer and Instituto de Biologıa Molecular y Celular del Cancer, Consejo Superior de Investigaciones Cientıficas (CSIC), University of Salamanca, Salamanca, Spain Abstract To establish latent infections in B-cells, gammaherpesviruses express proteins in the infected B-cells of the host that spuriously activate signalling pathways located downstream of the B-cell receptor. One such protein is M2, a murine gammaherpesvirus 68-encoded molecule that activates the Vav1/Rac1 pathway via the formation of trimolecular complexes with Scr family members. Previous reports have shown that the formation of this heteromolecular complex involves interactions between a proline rich region of M2 and the Vav1 and Fyn SH3 domains. Here, we s