uncovering suitable reference proteins for expression studies in human adipose tissue with relevance to obesity发现合适的参考蛋白表达在人类脂肪组织与肥胖的相关性研究.pdfVIP
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uncovering suitable reference proteins for expression studies in human adipose tissue with relevance to obesity发现合适的参考蛋白表达在人类脂肪组织与肥胖的相关性研究
Uncovering Suitable Reference Proteins for Expression
Studies in Human Adipose Tissue with Relevance to
Obesity
´ ´ 1,2 ´ 3 ´ 1,2 3 ´ ´ 1,2
Rafael Perez-Perez , Juan A. Lopez , Eva Garcıa-Santos , Emilio Camafeita , Marıa Gomez-Serrano ,
4,2 4,2 ´ ´ 4,2 ´ 1,2
Francisco J. Ortega-Delgado , Wifredo Ricart , Jose M. Fernandez-Real , Belen Peral *
´ ´ ´
1 Instituto de Investigaciones Biomedicas, Alberto Sols, Consejo Superior de Investigaciones Cientıficas (CSIC) Universidad Autonoma de Madrid (UAM), Madrid, Spain,
´ ´ ´
2 CIBER Fisiopatologıa de la Obesidad y Nutricion (CIBERobn), ISCIII, Madrid, Spain, 3 Unidad de Proteomica, Centro Nacional de Investigaciones Cardiovasculares (CNIC),
Madrid, Spain, 4 Department of Diabetes, Endocrinology and Nutrition, Hospital Dr. Josep Trueta, Girona, Spain
Abstract
Background: Protein expression studies based on the two major intra-abdominal human fat depots, the subcutaneous and
the omental fat, can shed light into the mechanisms involved in obesity and its co-morbidities. Here we address, for the first
time, the identification and validation of reference proteins for data standardization, which are essential for accurate
comparison of protein levels in expression stud
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