zyfish a simple, rapid and reliable zygosity assay for transgenic micezyfish一个简单、快速、可靠的转基因老鼠接合性试验.pdfVIP

ZyFISH: A Simple, Rapid and Reliable Zygosity Assay for Transgenic Mice Donal McHugh, Tracy O’Connor, Juliane Bremer, Adriano Aguzzi* Institute of Neuropathology, Department of Pathology, University Hospital of Zurich, Schmelzbergstrasse, Switzerland Abstract Microinjection of DNA constructs into fertilized mouse oocytes typically results in random transgene integration at a single genomic locus. The resulting transgenic founders can be used to establish hemizygous transgenic mouse lines. However, practical and experimental reasons often require that such l